【摘要】 目的:探讨低氧诱导因子1α(HIF-1α)与神经细胞缺氧复氧损伤的关系。方法:氯化钴处理PC12细胞模拟缺氧,去除氯化钴模拟复氧,用乳酸脱氢酶(LDH)试剂盒和RT-PCR技术检测缺氧0、1、2、4、8、12、16h以及复氧4、8、12hLDH漏出与HIF-1αmRNA水平。结果:①氯化钴处理细胞后,LDH漏出逐渐增加,8h达高峰,随后逐渐下降;HIF-1αmRNA水平逐渐增加,4h达高峰,随后逐渐下降。②氯化钴处理4h,去除氯化钴后,LDH漏出于8h明显增加,HIF-1αmRNA表达于4h明显减少,8、12h略有升高。结论:HIF-1α对神经细胞缺氧复氧损伤具有保护作用。更多还原

【Abstract】 Objective:To study the relationship between hypoxia inducible factorl α and hypoxia/reoxygenation injury on PC12 cells. Methods:Hypoxia injury was prepared in PC12 cells which were managed with cobalt chloride;reoxygenation injury was prepared in PC12 cells with removal of cobalt chloride. Lactic dehydrogenase(LDH) in culture medium and HIF-1 α mRNA were measured at 0、1、2、4、8、12 and 16 hours after hypoxia and at 4、8、12 hours after reoxygenation. Results:After hypoxia the leakage of LDH was gradually increased,peaked at 8 h,and then decreased gradually;the expression of HIF-1α mRNA was also increased,peaked at 4 h,then decreased. After reoxygenation the leakage of LDH was dramatically increased at 8 h,the expression of HIF-1α mRNA was decreased at 4 h reoxygenation,increased a bit at 8 or 12 h. Conclusion:HIF-1α may protect PC12 cells from hypoxia/reoxygenation injury,however the exact action and mechanism of HIF-1α should be studied further.更多还原