【摘要】 应用以明胶为底物的SDS 聚丙烯酰胺凝胶电泳技术对 4 8例乳腺癌术后患者尿液进行酶谱分析 ,探讨对乳腺癌术后患者行尿液MMPs检测的临床意义 .结果显示乳腺癌患者尿中可检测到 3种分子量分别为 130kD、92kD、72kD的MMPs .乳腺癌术后复发转移组尿中 130kD、92kD、72kD的MMPs检出率分别为 80 .8%、84 .6 %及 6 9.2 % ,而术后无转移组的 3种MMPs检出率分别为 2 7.3%、31.8%及 2 2 .7% ,正常对照组的检出率分别为 13.3%、2 0 .0 %及10 .0 % ,乳腺癌术后复发转移组尿液MMPs检出率明显高于术后无转移组 ,P <0 .0 1.而乳腺癌术后无转移组尿MMPs的检出率与正常对照组无显著性差异 .上述结果提示用酶谱电泳技术检测乳腺癌患者尿液MMPs可用于乳腺癌术后复发转移的监测更多还原

【Abstract】 Matrix metalloproteinases (MMPs) have been implicated in mechanism of metastasis in human malignancies. We used substrate gel electrophoresis to detect MMPs in the urine of 48 breast cancer patients after operation. Three molecular weight classes of urinary MMPs, M r 130 kD, 92 kD and 72 kD species, were detected. The posive rates of thress MMPs in the urine of the metastatic breast cancer group were 80.8%, 84.6% and 69.2%, respectively, which were much higher than those (27.3%, 31.8% and 22.7%, respectively) in the urine of the non_metastatic cancer group, and also higher than those (13.3%, 20.0% and 10.0%, respectively) in the urine of the normal group. The data suggested that the detection of MMPs in the urine of breast cancer after operation using zymography was an important predict or of metastatic breast cancer.更多还原