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脱水MS培养基及其在马铃薯脱毒试管苗中的应用
PREPARATION OF MS DRY MEDIUM AND ITS APPLICATION IN THE CULTURE OF VIRUS-FREE POTATO PLANTLETS IN VITRO
【摘要】 采用浸润分配及糖包盐隔离工艺 ,以“倍力凝”代替琼脂 ,制备出脱水MS培养基 ,以马铃薯脱毒试管苗Mela为检验品种 ,比较了脱水MS培养基与标准MS培养基对Mela生长的影响 ,结果表明 ,脱水MS培养基达到了标准MS培养基的培养效果 ,而且还具有生长快、根系发达、茎叶粗壮等优势。
【Abstract】 Using "PolyGel"instead of agar,MS dry medium has been prpared by "salt in sucrose granulation"and "spray soaking distribution"methods.By using virus free potato plantlets in vitro of cv "mela",the growth effects of this dry medium have been compared with that of the standard MS medium.The results showed that this dry medium provided better growth,especially stronger roots,and stronger stems and foliages.$$$$
【关键词】 脱水MS培养基;
马铃薯;
脱毒苗;
应用;
【Key words】 MS dry medium; potato; virus free plantlet in vitro; aplication;
【Key words】 MS dry medium; potato; virus free plantlet in vitro; aplication;
- 【文献出处】 中国马铃薯 ,Chinese Potato , 编辑部邮箱 ,2002年05期
- 【分类号】S532
- 【被引频次】2
- 【下载频次】132