【摘要】 本研究采用MS培养基 ,附加不同浓度的活性炭、6 BA、NAA ,按正匀表L9(34 )前 3列形成 3因素、3水平的不同配比 ,比较了银杏茎段试管培养的植株再生效果 ,分析了不同培养基中不同激素浓度配比及有无活性炭对茎段分化的影响 ,并对取自不同阶段不同生长期的银杏茎段的植株再生能力进行了比较。试验结果表明 ,以MS培养基 +活性炭 0 5 % +6 BA1 0mg·L- 1 +NAA0 1mg·L- 1 配方对茎段分化和植株再生效果最好 ;各因素中 ,活性炭的正向效果最显著 ;取材时间以 4月中、下旬为佳更多还原

【Abstract】 The tissue culture of the stem section in Ginkgo biloba carried out in the paper,using MS medium with the addition of different concentrations of activated carbon,6\|BA and NAA.The orthogonal table L\-9(3\+4)with the compound of three elements with three levels was selected.The various actions on the culture effect were analysed,such as different hormone,including activated carbon or not,and the regeneration ability of stem section in different growth period.The results showed that the MS medium with activated carbon 0.5%+6\|BA1.0?mg·L -1 +NAA 0 1?mg·L -1 was the best for the culture of stem section in Ginkgo biloba. In all elements,the positive effect of activated carbon was the most obvious.The proper time of sampling was in the middle and last ten days of April.更多还原