【摘要】 目的 :观察参麦注射液对缺血再灌注性鼠肾小管细胞损伤的作用。方法 :用传代培养的肾小管细胞复制缺血再灌注模型 ,MTT法测定细胞增殖活性 ,酶组化法测定细胞膜和线粒体中ATP酶和LDH含量的变化 ,用生化法检测小管细胞内SOD活性和MDA含量 ,观察参麦注射液 (SMI)对培养的鼠肾小管细胞的增殖和对缺血再灌注性小管细胞损伤的作用。结果 :不同浓度的SMI对肾小管细胞有明显的促增殖作用 ,在SMI浓度为 5 0 μg .ml- 1 时 ,促增殖作用最强 ;SMI能显著提高IR后小管细胞的增殖活性和增加细胞的存活率。缺血培养 4小时后 ,MDA含量明显增高 ,ATP酶、LDH含量明显降低 ,SMI降低IR培养的小管细胞MDA含量 ,增强SOD活性 ,ATP酶、LDH含量。结论 :本实验提示SMI具有促进鼠肾小管细胞增殖和减轻肾小管细胞IR损伤的作用 ,其机制可能与增强SOD的活性、加速OFR的清除 ,增强细胞抗氧化的能力 ,减轻膜的脂质过氧化 ,抑制ATP酶含量减少 ,进而持续生物膜的结构和功能的完整性有关。更多还原

【Abstract】 Objective: To investigate the effect and mechanism of SMI on stimulating the proliferation of cultured human tubular cell and relieving the injury induced by ischemic reperfusion.Methods:From the cultured human renal tubular cell strain the IR model was made and the drugs were tested. The proliferative activity of tubular cell was measured with the method of MTT. The content of ATPase and LDH on cell membrane and mitochondrion were detected by the histochemistry method. The activity of SOD and the content of MDA were detected by biochemistry method.Results:The effect of stimulating proliferation of normal tubular cell was remarkable and this effect reached a peak in SMI 50μg.ml -1 . The proliferative activity and the cell survial rate in IR+SMI group were much higher than those in IR group. The activity of SOD in IR+SMI group was much higher than that in IR group, the content of MDA was much lower than that in IR control group, All were significantly different. The content of ATPase in IR+SMI group markedly increased compared with IR group. Conclusion:All the data indicated that Shenmai injection could accelerate the proliferation of tubular cell and relieve the injury resulting from ischemia-reperfusion. A possible mechanism could be related with the increased ability of renal cell to clear OFR, the protection of membrane lipids from peroxidation, and the membrane Na +-K + ATPase and LDH could be prohibited from decreasing,so that the structure and function of tubular cells stay intact. All these may provide a theoretical basis for the clinical treatment of acute renal ischmiareperfusion injury.更多还原