【摘要】 目的　研究丹参素对豚鼠心室肌细胞膜L 型钙通道的影响 ,探讨丹参素在离子通道水平的药理机制。方法　急性酶解法获得豚鼠的单个心肌细胞 ,用标准的全细胞膜片钳技术记录钙电流。结果　在保持电位 (Holdingpoten tial,HP)为 - 80mV ,预先去极化至 - 4 0mV ,再去极化至0mV ,波宽为 30 0mS的刺激参数下 ,可记录到一具有电压和时间依赖性内向的电流 ,当用含 1μmol/L硝苯地平的台氏液灌流时该电流被迅速消除 ,在灌流液中加入丹参素 1mg/mL和 10mg/mL ,1mg/mL组对钙电流无明显改变 (P >0 0 5 ,n =5 ) ,10mg/mL组使钙电流减少 [- (7 76± 0 85 )pA/pFvs - (2 6 2 6± 0 5 9)pA/pFP <0 0 5 ,n =8],并使心肌细胞钙电流 -电压曲线上移 ,原有的电流 -电压依赖特征不变。结论　丹参素浓度依赖性地抑制心肌L 型钙通道更多还原

【Abstract】 Objectives To determine the effects of Danshen on L type calcium channel in isolated ventricular myocytes of guinea pig. Methods Using whole cell patch clamp recording technique.Results The nifedipine sensitive L type calcium inward current(ICa) was recorded in the ventricular myocytes of guinea pig. Danshen at 10mg/mL obviously decreased peak ICa 66.23%( p<0 05,n=8 ) in the extracellular respectively solution, Danshen at 1 mg/mL hadn’t significant ( p>0 05,n=5 ).We observed no effects of Danshen on the activation and inactivation curves of ICa.Conclusions Danshen concentration dependently prohibited L type calcium channels in ventricular myocytes of guinea pig.更多还原