【摘要】 目的　探讨肾移植受者术前分泌TNF α、IL 10的能力 ,预测术后急性排斥反应的意义。方法　采用酶联免疫吸附试验 (ELISA)方法 ,术前检测 35名肾移植受者体外培养的外周血及单个核细胞产生的TNF α、IL 10水平。结果　术后发生急性排斥反应的 7例 ,未发生急性排斥反应的 2 8例。发生排斥反应的受者体外TNF α分泌量为 5 4 6 .84± 12 0 .35pg ml,未发生组平均为 2 6 7.2 3± 31.5 6pg ml,两组间有显著差异 (P <0 .0 0 1)。IL 10水平在发生与未发生急性排斥反应组分别为 4 5 1.86± 16 9.93pg ml、32 0 .5 7± 4 8.78pg ml,两组间亦有统计学差异 (P <0 .0 5 )。结论　术前检测肾移植受者分泌TNF α、IL 10的能力 ,有助于预测发生急性排斥反应的危险性。更多还原

【Abstract】 Objective To study the secretion ability of tumor necrosis factor alpha and interleukin-10 before operation predicting acute graft rejection following renal transplantation. In 35 preoperative recipient, we measured TNF αand IL 10 production of peripheral blood cells and mononuclear cell by enzyme linked immunosorbent assay. Results After operation 7 cases experienced acute rejection and 28 cases didn’t. The quantity of TNF αthat recipients experiencing acute rejection produced in vitro is 546.84±120.35pg/ml, the quantity produced by recipients not experiencing acute rejection is 267.23±31.56pg/ml, two groups showed significant difference(P<0.001). The level of IL 10 with or without acute rejection group is respectively 451.86±169.93pg/ml?320.57±48.78pg/ml,two groups also had statistics significance(P<0.05). Conclusion Detecting the secretion ability of TNF αand IL 10 before operation can help to predict the risk of acute rejection following renal transplantation.更多还原