【摘要】 目的 :探讨我国男性原发性高血压 (EH)患者及其危险分层与血管紧张素Ⅱ的 2型受体 (AT2 R)基因C312 3A多态性的关系。方法 :①随机选取 85例男性EH患者 ,另选 4 5例男性正常人作为对照。②用聚合酶链式反应 (PCR) /限制性内切酶AluI酶解检测AT2 R基因的C312 3A多态性。③根据心血管绝对危险水平分层把患者分为低中危、高危和极高危者。结果 :高危和极高危者的AT2 R基因的A等位基因频率均明显高于低中危者 (P <0 .0 5 ) ,但AT2 R基因的A和C等位基因频率与正常对照组间的差别却无统计学意义 (P >0 .0 5 )。结论 :AT2 R的C312 3A基因多态性与男性EH发病无关 ,但突变的A等位基因与EH患者的靶器官损害及并存的临床情况有密切关系更多还原

【Abstract】 Objective:To investigate the association between polymorphism of angiotensin type 2 receptor (AT 2R) gene and essential hypertension (EH).Method:① Genomic DNA was extracted from peripheral blood leukocytes of 85 male hypertensive patients and 45 normal male controls. ② The C3123A polymorphism of AT 2R gene was detected by PCR/AluI restricton endonuclease digestion. ③ By the clinical absolute cardiovascular risk level the patients were divided into low moderate risk group, high risk and extremely high risk groups. Result: In male EH group including high risk and extremely high risk groups, the frequencies of A allele of AT 2R gene C3123A polymorphism were significantly higher than those in low moderate risk group (P< 0.05 ); and there was no significantly difference about the frequencies of A and C alleles between foregoing groups and male control group (P> 0.05 ). Conclusion:The C3123A polymorphism of AT 2R gene is not associated with male EH occurrence, but mutative A allele is involved in target organ damages and concurrent clinical conditions.更多还原