【摘要】 目的　探讨端粒酶抑制剂对膀胱癌 BIU- 87细胞端粒酶活性的影响。方法　用TRAP- EL ASA法检测端粒酶抑制剂反义硫代寡核苷酸 (as ONS)对膀胱癌 BIU- 87细胞及其细胞裂解物端粒酶活性的影响。结果　 as ONS能够部分抑制人膀胱癌 BIU- 87细胞端粒酶的活性 ,呈现一定的浓度依赖性和时间依赖性 ;as ONS与细胞裂解物直接作用组同作用细胞组相比 ,端粒酶活性下降的更为明显。结论　 as ONS如何有效通过细胞膜将是保证疗效的关键更多还原

【Abstract】 Objective To detect the Telomerase inhibitor-antisense phosphorothiate oligonucleotide (asONS) effect on telomerase activity of human bladder tumor cell line BIU-87.Methods BIU-87 cells and cell extracts are cultured with asONS,non-asONS and control fluid at different concentrations and times respectively.Telomerase activity was assayed by TRAP PCR-ELASA.Results AsONS can inhibit the activity of BIU-87 cells ,whereas non-asONS and control group not, and have significant difference(P<0.05).Compared with that of cells group, asONS has greater effect on the activity of cell extracts group, there is significant difference between two groups.Both of asONS take on dose/time-effect dependent relation. Conclusions AsONS could inhibit the telomerase activity of BIU-87 cells and shows dose/time-effect dependent relation. They have greater effects on telomerase activity of cells extracts than that of cells. It suggests that the key to ensure the efficacy is how to make drugs pass the cell membrane efficiently.更多还原