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甘油处理的同种异体神经在临床的应用

The clinical application of nerve allograft processed with glycerol

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【作者】 刘金慕彭昊王志林

【Author】 LIU Jinmu,PENG Hao, WANG Zhilin.Department of Orthopacdic Surgery, Renmin Hospital of Wuhan University,Wuhan 430060,China

【机构】 武汉大学人民医院骨科武汉大学人民医院骨科 430060430060

【摘要】 目的 寻找一种制作简便 ,保存方便同时又无明显抗原性 ,具备完整神经内膜结构 ,并能长期保留的神经移植物。方法 将无菌条件下取得的同种异体神经在 3 7℃条件下 ,依次在5 0 %、70 %、85 %的甘油中各浸泡 3h ,再分装在盛有 85 %甘油容器中密封 ,置于 5℃保存 6周后备用。结果  10例患者 ,行甘油处理后的同种异体神经移植 14条 ,长度为 2 .5~ 18cm ,术后经过 5~42个月的随访观察 ,获得满意效果。结论 同种异体神经通过甘油处理贮存 6周后 ,用于桥接周围神经缺损 ,有明显效果 ,是一种值得进一步研究应用的神经移植材料。

【Abstract】 Objective To find out easily-processed and longly-preservated nerve allograft transplantation which has not only no-obvious antigenicity,but the integral structure of intermal nerve membrane.Mehtods Acquire the nerve allografts under sterile condition,submerge them into 50 ,70 and 85 percent of glycerol for 3 hours respectively at 37 Centigrade degree,then put them into the containers which contain 85 percent of glycerol. After sealed and preservated at 5 Centigrade degree for 6 weeks,the processed nerves can be used for transplantation.Results 14 nerve allografts processed with glycerol were transplanted in 10 patients with nerve defects varying from 2.5 to 18 cm. After follow-up for 5 to 42 months,the effects were proved to be satisfactory.Conclusion It is obviously effective to transplant nerve allograft processed with glycerol and preservated for 6 weeks for bridging the defects of peripherall nerve through a series of clinical observations.

【关键词】 甘油异体神经移植
【Key words】 GlycerolNerve allograftTransplantation
  • 【文献出处】 临床外科杂志 ,Journal of Clinical Surgery , 编辑部邮箱 ,2002年04期
  • 【分类号】R651.3
  • 【被引频次】18
  • 【下载频次】34
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