【摘要】 目的　研究癌性胸水细胞端粒酶活性的检测意义。方法　采用端粒重复序列扩增 -核酸杂交分析法检测癌性胸水细胞端粒酶活性 ,并将检测结果与细胞学诊断进行比较。结果　 4 9例诊断明确的癌性胸水细胞样本中 2 3例细胞学阳性胸水有 2 0例端粒酶阳性 ,5例细胞学可疑胸水有 4例端粒酶阳性 ,2 1例细胞学阴性有 14例端粒酶阳性。细胞学诊断和端粒酶检测总阳性率分别为 4 6 .9% (2 3/ 4 9)和 77.6 % (38/ 4 9) ,二者联合检测率为 83.7% (41/ 4 9)。结论　胸水细胞端粒酶活性的检测有助于癌性胸水的诊断 ,弥补胸水细胞学诊断的不足更多还原

【Abstract】 Objective To investigate the telomerase activity in malignant pleural effusion cells.Method Forty nine specimens of pleural effusion cells from patients with maligant neoplasms were examined for telomerase activity by TRAP( telomeric repeat amplification protocol) Hyb(Hybrid)method,and the results of detecting telomerase activity was compared with the cytological diagnosis.Results Telomerase activity was detected in pleural effusion cells from patients with malignant neoplasms in 20 of 23 cytologyical positive specimens and in 4 of 5 cytologic suspicious spcimens and in 14 of 21 cytoligic negative specimens.The overall positive rate in 49 malignant pleural effusion cells samples by cytological examination and by the detection of telomerase activity is 46.9%(23/49) and 77.6%(38/49) and the combined diagnosis rate is 83.7%(41/49).Conclusions The results indicate that the measuring telomerase activity of pleural effusion cells may remedy the insufficiency of the cytological examination in malignant pleural effusion.更多还原