【摘要】 目的:研究增殖细胞核抗原(PCNA)与表皮生长因子受体(EGFR)在人脑星形细胞瘤中的表达及与星形细胞瘤病理分级的关系。方法:利用免疫组化方法检测44例星形细胞瘤病理蜡块中PCNA与EGFR蛋白的表达水平并比较二者的相关性及其与星形细胞瘤病理分级的关系。结果:PCNA表达强度随星形细胞瘤病理级别的升高而升高,两者呈显著正相关(r=0.8801,P<0.05)。Ⅲ~Ⅳ级与Ⅰ~Ⅱ级、正常脑组织相比,差异有显著性(P<0.01)。随着星形细胞瘤病理级别的升高EGFR蛋白的表达率逐渐升高,Ⅰ~Ⅳ级阳性率分别为11.1%、33.3%、63.6%、75.0%。二者之间呈正相关(r=0.6597,P<0.05)。Ⅲ~Ⅳ级与Ⅰ~Ⅱ级、正常脑组织相比,差异有显著(P<0.01)。而PCNA的评分与EGFR的表达状况无显著相关性(χ2=17.11,P>0.05)结论:PCNA与EGFR的免疫组化检测有利于判断星形细胞瘤的发生发展、恶性程度及增殖性,为临床上对该肿瘤的诊断、鉴别诊断、治疗及预后判定提供了重要的参考指标更多还原

【Abstract】 Objective: To study the expression of PCNA and EGFR in human brain astrocytomas and the relationship between the expression rate and pathological grade in astrocytomas. Methods: The expression levels of PCNA and EGFR in 44 cases of humen astrocytomas pathlogical paraffin were detected by immunohistochemical technics, The correctations between PCNA, EGFR and the histological grade of astrocytomas were analysed respectively. Results: he expression of PCNA increased with the increasing of pathological grade,and there was a positive correlation(r=0.8801, P<0.05). There was a significant difference between Ⅲ~Ⅳ grades, Ⅰ~Ⅱgrades and the normal brain constitution(P<0.01). With the increasing of pathological grade, the expression rate of EGFR increased gradually, the positive rates in grade Ⅰ,Ⅱ,Ⅲ and Ⅳ were 11.1%,33.3%,63.6% and 75.0% respectively, and there was a positive correlation between pathological grade and EGFR. (r=0.6597, P<0.05). There was a significant difference between Ⅲ~Ⅳ grades,Ⅰ~Ⅱgrades and the normal brain constitution. (P<0.01). but there was no significant relationship between PCNA and EGFR. (χ2=17.11, P>0.05). Conclusions: The immunohistochemistry detection of PCNA and EGFR takes advantage of judging the development,progress, malignancy and proliferation of astrocytomas and provides a useful reference mark for diagnosing,differential diagnosing, treating and evaluating prognosis of the tumour in clinic.更多还原