【摘要】 通过优化得出了耐热酿酒酵母 HU- TY- 1及其亲株 LK的最佳产孢条件 .利用随机孢子分析获得了 HU- TY- 1的单倍体 Hs系列和 LK的单倍体 Ls系列 ,并对它们进行了发酵效率的测定 ,发现 Hs系列在 40℃条件下发酵效率好于 Ls系列 ,体现了耐高温特性 .此外对由 HU- TY- 1及其单倍体 Hs1 2 5自身融合构建的二倍体与四倍体菌株进行发酵效率的分析显示 ,随着核倍性的增加 ,菌株的耐热性有增加的趋势 .更多还原

【Abstract】 The optimum sporulation conditions for thermatoleant strain HU TY 1 and original strain LK was confirmed by optimization test. The haploid strains of lines Ls and Hs from LK and HU TY 1 were acquired, and fermentation activity of them was analyzed.The result showed that the fermentation activity of line Hs was high than that of line Ls at 33℃ and 40℃. In addition to protoplast self fusion of Hs125 and HU TY 1 were performed, and the fusants of diploid and tetraploid were obtained. The analysis of fermentation activity of them showed the fermentation activity enhanced by the nuclear multiploidy increasing.更多还原