【摘要】 目的 :调查我国不同地区、通过不同传播途径感染人类获得性免疫缺陷病毒I型 (HIV 1)患者中丙型肝炎病毒(HCV)的流行情况及不同亚型的分布。方法 :采用酶联免疫吸附试验 (ELISA)检测抗 HIV 1并以蛋白印迹试验 (Westernblot,WB)进行确认。采用DNA分支放大 (bDNA)技术检测HIV 1病毒载量 ,采用荧光抗体流式细胞检测技术 (FACs)作CD4和CD8细胞计数。抗 HCV检测采用ELISA方法。HCV基因亚型的测定采用实时 (real time)聚合酶链反应 (PCR)方法。结果 :共检测了 2 39例HIV 1感染者 ,抗 HCV阳性率为 5 6 .9%(136 / 2 39) ,其中经不同传播途径感染HCV的阳性率分别为 :静脉注毒 :42 .7%(5 8/ 136 ) ;经血液 :5 3.7%(73/ 136 ) ;性接触途径 :3.7%(5 / 136 )。静脉注毒者 (云南和新疆 )HCV感染以 1,3,4亚型最多 ,而输血人群 (河南省 )感染的HCV以 1,2亚型为主。结论 :HIV 1感染者中存在HCV混合感染 ,我国HCV基因亚型以 1型为主。更多还原

【Abstract】 Objective: To investigate the prevalence and subtypes of HIV-1 and HCV in different groups of HIV-1 infected patients in China. Methods:HIV infection was detected using ELISA and confirmed by Western blot. HIV-1 viral load was measured by branched-DNA (bDNA) method. CD4 and CD8 cells count was measured by FACS. Anti-HCV was detected by ELISA method, and HCV genotypes were tested using real time PCR. Results:Of 239 HIV-1 infected patients, 56.9% (136/239) were HCV antibody positive. The majority of HCV co-infection is associated with intravenous drug using (42.7%) and illegal blood donation (53.7%). HCV co-infection through sexual contact, however, is approximately 3.7%. Genetic characterization of the viral sequences indicates that the illegal blood donors in Henan carry HIV-1 subtype B and HCV genotypes 1 and 2, whereas those intravenous drug users in Xinjiang and Yunnan carry HIV-1 subtype C and HCV genotypes 1, 3, and 4. Conclusions: Our findings show that the situation of HIV-1 and HCV co-infection in China is serious. HCV genotype 1 is the dominant subtype in China.更多还原