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利用mtDNA COⅠ基因序列鉴定我国烟粉虱的生物型
The use of mitochondrial cytochrome oxidase Ⅰ(mt COⅠ)gene sequences for the identification of biotypes of Bemisia tabaci(Gennadius)in China
【摘要】 利用mtDNACOⅠ基因片段作标记 ,采用序列分析的方法 ,从分子生态学的角度研究了近年来在我国暴发危害的烟粉虱Bemisiatabaci 5个种群 (北京一品红种群 ,广州甘蓝种群 ,西安一品红种群 ,北京西红柿种群和新疆吐鲁番棉花种群 )的生物型。在 5个种群的基因片段上 ,截取与Texas B型相应的 72 0bp的序列分析 ,结果表明所测序列中只有 2个碱基与Texas B型不同 ,序列相似性为 99 7% ;在西安一品红种群和新疆吐鲁番棉花种群的原序列中截取与Arizona B型序列相应的4 2 3bp片段 ,分析表明这两个种群与AZB3型属于同一个单倍型。因此 ,我国烟粉虱 5个实验种群的生物型与Texas B型和Arizona B型种群为同一生物型“B”。
【Abstract】 Mitochondrial cytochrome oxidase Ⅰ (mt COⅠ) (~830?bp) gene sequences were utilized as markers to identify biotypes of 5 populations of Bemisia tabaci in China. Of the 720 characters examined for COⅠ sequences, only 2 bases exhibited divergence and overall similarity was 99 7%. Examination of 423 characters in populations from Xian and Tulufan revealed that these two populations were of the same haplotype as AZB3. The results indicated that all 5 populations were of the B biotype.
- 【文献出处】 昆虫学报 ,Acta Entomologica Sinica , 编辑部邮箱 ,2002年06期
- 【分类号】S433.3
- 【被引频次】442
- 【下载频次】1056