【摘要】 用PCR方法检测了采集于不同地域稻田的 3种稻飞虱共生菌沃尔巴克氏体 (Wolbachia)的感染 ,发现灰飞虱Laodel phaxstriatellus、褐飞虱Nilaparvatalugens、白背飞虱Sogatellafurcifera为沃尔巴克氏体所感染。克隆了编码沃尔巴克氏体外膜蛋白质的wsp基因并进行了序列测定。对wsp的RFLP分析证实了这些飞虱为单一沃尔巴克氏体感染。研究了灰飞虱中沃尔巴克氏体所诱导的胞质不相容性及其在不同地域灰飞虱中的分布。还发现能寄生于上述 3种飞虱的稻虱红螯蜂也受同种沃尔巴克氏体感染。沃尔巴克氏体可能通过这种寄生蜂在不同昆虫间横向传播更多还原

【Abstract】 The infection of the bacterial symbionts Wolbachia in 3 planthoppers was detected by PCR method. Laodelphax striatellus, Nilaparvata lugens and Sogatella furcifera were found to be infected with Wolbachia. The gene wsp, encoding the outer membrane protein of the symbiotic bacteria, was cloned and sequenced. The RFLP analysis confirmed that those planthoppers were infected with the single Wolbachia strain. The cytoplasmic incompatibility (CI) induced by Wolbachia in Laodelphax striatellus and the distribution of Wolbachia in this insect from different regions were studied. A parasitic wasp, Haplogonatopus apicalis, which can parasitize the planthoppers, was found to be infected with the same Wolbachia. Wolbachia might transfer between different insect horizontally via this wasp.更多还原