【摘要】 目的 :观察黄芪注射液对大鼠烫伤后肠源性感染的影响 ,给药剂量及时间对药效的影响 ,并初步研究其作用机制。方法 :采用大鼠 40 %TBSAIII度烫伤模型 ,腹腔注射黄芪注射液 0 .2ml/ 10 0g、0 .4ml/ 10 0 g ,分别进行预防性3d给药、治疗性 3d、5d给药 ;活杀动物 ,进行 (1)肠粘膜组织学分析 ;(2 )肠系膜淋巴结 (MLN)、肝、脾匀浆细菌培养 ;(3)血清内毒素检测 ;(4 )肠粘膜MDA检测 ;(5 )存活率观察。结果 :烫伤后大鼠存活率降低 ,细菌大量移位于肠系膜淋巴结 (MLN)、肝、脾 ,血清内毒素值明显增加 ,肠粘膜结构受损 ,肠粘膜MDA含量增加 ;黄芪注射液大剂量组可增加大鼠存活率 (P <0 .0 5 ) ,但小剂量无明显影响 ;大、小剂量组均能显著降低肠道细菌移位 (P <0 .0 5 ) ,对血清内毒素虽有减少趋势 ,但无统计学差异 (P >0 .0 5 )。黄芪注射液对烫伤后肠粘膜结构有明显改善作用 ,肠粘膜MDA含量也较烫伤组减少 (P <0 .0 5 )。黄芪小剂量组对细菌迁移的抑制作用较大剂量组有更强趋势 ,但无统计学意义 (P >0 .0 5 )。黄芪注射液预防性 3日给药组对以上指标无明显影响 ,5d治疗性给药组较 3d给药组疗效有更强趋势 ,但无统计学意义 (P >0 .0 5 )。结论 :黄芪注射液对烫伤大鼠肠道细菌迁移有抑制作用 ;黄芪注射液可改善烫伤?更多还原

【Abstract】 Objective:To observe the effect of Astragalus Membranacenus (A.S.) Injected solution on intestine-derived infection in burned rats and its mechanism.Methods:Rats were scalded for 12 seconds with 99℃ hot water to achieve a 40% total body surface area (TBSA) deep partial-thickness burn. The rate in treatment group were injected intraperitoneum with A.S at a dose of 0.2 ml/100g/d or 0.4 ml/100g/d,and were sacrificed for following examinations after 3 days or 5 days administration:(1)Morphological examination;(2)bacterial culture to homogenization of mesenteric lymphatic nodes(MLN),liver,spleen depathogenizedly;(3)Value of plasma endotoxin;(4)Examination of MDA in intestine,(5)Analysis of survival rate of scalded rats.Results:The survival rate decreased in burned group, but increased in the group of large-dose A.M administrated for 5 days(P<0.05).A great number of bacterial were found in MLN,liver,and spleen.Bacterial counting decreased in A.M.group,compared to control group as well as MDA value.Although the value of endotoxin was less in treatment group than that in control group,there was no statistical significance(P>0.05).The construction of intestinal mucous was damaged apparently including shortening of villi and loss of mucosal mass,however,this trend was reversed in A.M group. It seemed that the above-mentioned indexes in rats administrated for 5 days achieved more improvement than those for 3 days.Even though,there was no statistical significance in these differences.Dose-effect relationship was different referring to different subjects.Conclusion:Astragalus Membranacenus injected solution could decrease the bacterial translocation of burned rats and it can rehabilitate the damaged construction of intestinal mucous.更多还原