【摘要】 目的 :从成人胰腺组织中分离并鉴定胰腺干细胞。方法 :利用含bFGF的有血清培养基分离、培养出具有高度增殖能力的细胞群 ,采用RT PCR技术检测其NestinmRNA及InsulinmRNA表达情况。同时诱导定向分化 ,观察其分化后的状态。结果 :从成人胰腺组织中分离出的条索状细胞具有高度增殖能力 ,其NestinmRNA表达阳性 ,而胰岛素InsulinmRNA表达阴性 ,分化后的细胞形成胰岛细胞团样结构。结论 :本研究得到的细胞具有高度增殖能力和分化成胰岛细胞的潜能 ,是来源于胰腺的胰腺干细胞。更多还原

【Abstract】 Objective:To isolate and identify the pancreatic stem cells from adult pancreas.Methods:We used incubation base containing bFGF and serum to isolate the self-renewal cells and to induce their differentiation to islet cells.RT-PCR was employed to detect the expressions of Nestin mRNA and Insulin mRNA. Results:The cable-shaped cells we obtained from pancreas had positive expression of Nestin mRNA and negative expression of Insulin mRNA. After differentiation, they formed islet-like cells in vitro.Conclusion:Our cell lines have powerful abilities to grow and differentiate; they are pancreatic stem cells.更多还原