A new method for preparation of template DNA for PCR from special plant materials

【摘要】 <正>A simple method for preparation of template DNA suitable for PCR amplification from herbarium samples and plant tissues rich in byproducts, e.g. polysaccha-rides, tannins, polyphenolic, and terpenoids compounds, is described. The total DNA from regular extraction procedure is absorbed by a small amount of glass powder and the final precipitation of glass powder is used directly as a template for PCR. Taking six plant taxa, including the herbarium specimens of Lythraceae collected from Namibia in 1957 and the silicon-dried leaf tissue from mangrove plants (Rhizo-phoraceae and Combretaceae) rich in by-products as examples, the PCR products, including nrDNA ITS regions and cpDNA rbcL gene, amplified following the regular and new methods respectively are compared. Our method provides a simple, rapid and economic approach to purify and prepare template DNA for PCR from special plant materials.更多还原

【Abstract】 A simple method for preparation of template DNA suitable for PCR amplification from herbarium samples and plant tissues rich in byproducts, e.g. polysaccha-rides, tannins, polyphenolic, and terpenoids compounds, is described. The total DNA from regular extraction procedure is absorbed by a small amount of glass powder and the final precipitation of glass powder is used directly as a template for PCR. Taking six plant taxa, including the herbarium specimens of Lythraceae collected from Namibia in 1957 and the silicon-dried leaf tissue from mangrove plants (Rhizo-phoraceae and Combretaceae) rich in by-products as examples, the PCR products, including nrDNA ITS regions and cpDNA rbcL gene, amplified following the regular and new methods respectively are compared. Our method provides a simple, rapid and economic approach to purify and prepare template DNA for PCR from special plant materials.更多还原