节点文献

The interaction between purple membrane and membrane lipid

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【Author】 HU KunshengInstitute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China (e-mail: huks1401@ sun5.ibp.ac.cn)

【摘要】 <正>Bacteriorhodopsin in purple membrane was reconstituted into different lipid vesicles. The effect of three different lipids on the structure and function of bacteri-orhodopsin in lipid vesicles was studied by the observation on freeze-fracture eletron microscopy, the rotational diffusion of bacteriorhodopsin in lipid vesicles, the measurement of absorption spectrum, and the absorbance change with time. For these prepared samples, the results showed that DMPC was the stable lipid environment of bacteriorhodopsin; egg-pc causeed the loss of retinal chromophore of bacteriorhodopsin and it was not reversible change, cholesterol could stabilize the bacteriorhodopsin in lipid environment, but it caused the aggregation of bacteriorhodopsin.

【Abstract】 Bacteriorhodopsin in purple membrane was reconstituted into different lipid vesicles. The effect of three different lipids on the structure and function of bacteri-orhodopsin in lipid vesicles was studied by the observation on freeze-fracture eletron microscopy, the rotational diffusion of bacteriorhodopsin in lipid vesicles, the measurement of absorption spectrum, and the absorbance change with time. For these prepared samples, the results showed that DMPC was the stable lipid environment of bacteriorhodopsin; egg-pc causeed the loss of retinal chromophore of bacteriorhodopsin and it was not reversible change, cholesterol could stabilize the bacteriorhodopsin in lipid environment, but it caused the aggregation of bacteriorhodopsin.

【基金】 Thiswork was supported by the National Natural Science Foundation of China (Grant No. 30170235);the Chinese Academy of Sciences, and the Wellcome Trust of UK.
  • 【文献出处】 Chinese Science Bulletin ,科学通报(英文版) , 编辑部邮箱 ,2002年07期
  • 【分类号】Q939.1
  • 【下载频次】15
节点文献中: 

本文链接的文献网络图示:

本文的引文网络