【摘要】 对米根霉AS3 .82 0及少根根霉AS3 .2 893进行了原生质体化及再生条件的研究。它们原生质体化及再生的最佳条件为 :米根霉AS3 .82 0 :蜗牛酶、溶菌酶与纤维素酶之比为 2∶1∶2 ,酶浓度为 2mg/mL ,在 3 0℃下酶解 3h ;少根根霉AS3 .2 893 :蜗牛酶、溶菌酶与纤维素酶之比为 3∶1∶1 ,酶浓度为 1mg/mL ,在 3 0℃下酶解 3h。稳定缓冲液为含 0 .7mol/LKCl的 0 .0 5mol/L的磷酸缓冲液 (pH为 6.0 )。在上述条件下 ,原生质体产量可达 1 .7× 1 0 6～ 2 .0× 1 0 6 个 /mL。更多还原

【Abstract】 R.oryzae AS3.820 and R. arrhizus AS3.2893 were studied in this paper. The optimum conditions for their protoplast formation and regeneration are described as follows: for R. oryzae, 2mg/mL snailase plus lysozyme and cellulase as combined enzyme system (2∶1∶2); for R. arrhizus, 1mg/mL snailase plus lysozyme and cellulase as combined enzyme system (3∶1∶1); using 0.05mol/L phosphate buffer (pH 6.0) which contains 0.7mol/L KCl;for 3 hours,at 30℃. Under these conditions, the protoplast yield is about 1.7×10\+6～2.0×10\+6/mL.更多还原