【摘要】 目的 :应用醋酸后大鼠结肠黏膜损伤后修复的病理过程。方法 :用 1%醋酸灌注Wistar大鼠结肠造成大鼠结肠损伤的模型 ,损伤的结肠分别于第 1、3、7、15天和第 2 0天用光镜和扫描电镜进行观察。取得的组织分别进行PCNA染色和HE染色 ,结肠隐窝用盐酸浸润消化法分离。结果 :黏膜及黏膜下层的损伤在第 1天和第 3天之间达到高峰 ,伴随着黏膜水肿、再生、坏死和炎症反应 ,第 5天～第 7天结肠陷窝基底部可见明显增生的细胞。黏膜水肿和炎症反应消退 ,黏膜萎缩和隐窝减少都在大约1周左右停止。 2周时 ,黏膜增厚 ,隐窝下三分之二出现许多分叉 ,3周左右黏膜恢复正常。结论 :黏膜隐窝损伤修复的过程是以隐窝分裂增殖的方式进行修复 ,3周左右黏膜恢复正常 ,隐窝基底部的分裂增殖区的细胞对损伤黏膜修复起着非常重要的作用。更多还原

【Abstract】 Objective The repairment process of injured rat colonic mucosa with acetic acid was studied.Method The colon of the Wistar rats was infused by 1% acetic acid through a tube.The injured colons were studied periodically on the 1st,3rd,7th,15th and 20th experimental days with light and scanning electron microscopy.Prepared specimens were paraffin sections stained with hematoxylin and erosin and stained with PCNA seperatively.Crypts were isolated with HCl digestion method.Results The peak of damage to the mucosal and submucosal layers occured between the 1st and 3rd days with edema,regeneration,necrosis and inflammation.The proliferative zone of the colonic crypt was observed at the crypt base.The edema and inflammation subsided and mucosal atrophy and crypt reduction remained at around 1 week.At 2 weeks,the mucosa became thicken and crypts showed many branches in their lower two thirds.The mucosa recovered to almost normal by 3 weeks.Conclusion Injuried colonic mucosa was repaired by the fission mechanism of colon crypt.The mucosa recovered to almost normal by 3 weeks.Cell at the crypt base played an important role in repairing the injured colonic mucosa.更多还原