【摘要】 通过对布氏杆菌脂多糖抗原、超声波破碎抗原和离心抗原进行筛选和优化 ,确定超声波破碎抗原为最佳抗原 ,首次建立了检测鹿布氏杆菌抗体的间接ELISA。对其特异性、敏感性和重复性进行试验 ,并与其他常规方法进行比较试验 ,结果表明 :该方法的特异性强、敏感性高和重复性好 ,明显优于其他常规方法更多还原

【Abstract】 Three Brucellosis antigens, namely lip polysaccharide antigen, ultrasound antigen and centrifugal antigen were prepared and tested. The results showed that ultrasound antigen was the best one. A enzyme-linked immunosorbent assay(ELISA)which had specificity,sensibitity and repetition for Brucellosis in deer was developed .更多还原