【摘要】 目的　探讨急性有机磷农药口服中毒患者体内的一系列自由基异常反应及其对患者机体损伤程度的关系。方法　采用独立样本对照设计 ,用分光光度比色分析法检测了 58例急性有机磷农药中毒患者 (AOPP)和 58例健康成人志愿者 (HAV)的血浆过氧化脂质 (LPO)、一氧化氮 (NO)、维生素C (VC)、维生素E (VE)、β 胡萝卜素 (β CAR)含量和谷胱甘肽 -S -转移酶 (GST)活性及红细胞超氧化物歧化酶(SOD)、过氧化氢酶 (CAT)、谷胱甘肽过氧化物酶 (GSH -Px)活性。结果　与HAV组各生化参数 (BP)平均值 (AV)比较 ,AOPP组血浆LPO、NO和GST的AV均升高 ,差异具有显著性意义 (P <0 0 0 1 ) ,血浆VC、VE、β CAR和红细胞SOD、CAT、GSH Px的AV均降低 ,差异具有显著性意义 (P <0 0 0 1 )。对 58例AOPP患者红细胞乙酰胆碱酯酶 (AChE)活性与上述各BP的直线回归和相关分析表明 ,随着AChE值的降低 ,LPO、NO和GST值逐渐升高 ,均呈直线负相关 (P <0 0 0 5) ,而VC、VE、β CAR、SOD、CAT和GSH -Px值逐渐降低 ,均呈直线正相关 (P <0 0 0 1 )。对 58例AOPP和 58例HAV用上述BP进行判别分析的结果提示 ,判别正确率均为 1 0 0 %。结论　急性有机磷农药口服中毒患者体内的一系列自由基反应病理性加剧 ,采用上述参数可提高判别分析的正确率。更多还原

【Abstract】 Objective To study the relationship between abnormal reactions of a series of free radicals in the patients with acute organophosphorus pesticide poisoning through ingestion and damages induced by free radical abnormal reactions.Methods Using an independent samples control design 58 patients with acute organophosphorus pesticide poisoning(AOPP) and 58 healthy adult volunteers(HAV) were investigated by measuring their levels of lipoperoxides(LPO),nitric oxide(NO),vitamin C(VC),vitamin E(VE) and β carotene(β CAR),and activity of glutathione-S-transferase(GST) in plasma as well as activities of superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase(GSH-Px) in erythrocytes(RBC) with spectrophotometric assays.Results Compared with the average values(AV) of every biochemical parameters(BP) in the HAV group,the AV of the LPO,NO and GST in plasma in the AOPP group were significantly increased( P <0 001),and the AV of the VC,VE,β CAR in plasma as well as SOD,CAT and GSH-Px in RBC in the AOPP group were significantly decreased( P <0 001).Linear regression and correlation analysis of RBC-acetylcholinesterase(AChE)value and above BP values for 58 AOPP patients showed significant negative correlation between the AChE and LPO,NO,GST( P <0 001～0 005),while there was all significant linear positive correlation between the AChE and VC,VE,β CAR,SOD,CAT,GSH-Px( P <0 001).The findings of the above BP used to make discriminant analysis for 58 AOPP and 58 HAV suggested that the correct rates of the discriminant analysis were all 100%.Conclusion The findings in this study suggested that a series of free radical reactions in patients with acute organophosphorus pesticide poisoning by ingestion was aggravated.The above biochemical parameters could greatly increase the accuracy rates of discriminant analysis for these patients . [更多还原