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He-Ne激光对金针菇SOD高产株的诱变效应

Induced Mutations of Varitety of High Yield of Flammulina Velutipes SOD by He-Ne Laser

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【作者】 李耀维冯文新张素梅

【Author】 LI Yao-wei, FENG Wen xing, ZHANG Su mei (Shanxi Agricultural Universty, Taigu 030801, Shanxi, China; 2.Shanxi Taigu Peoples Hospital, Taigu 030800, Shanxi, China)

【机构】 山西农业大学山西省太谷县人民医院 中国山西太谷030801中国山西太谷030801中国山西太谷030800

【摘要】 用 He- Ne激光辐照诱变金针菇 (Flammulina Velutipes)的原生质体、菌丝体片段 ,分生孢子悬液 ,并进行初筛、复筛及突变株遗传稳定性研究。结果表明 :采用原生质体进行诱变 ,其正突变率 ,单株 SOD产量提高率 ,产SOD遗传稳定性均高于菌系体与分生孢子。激光诱变原生质体是获得金针菇 SOD高产株的有效途径

【Abstract】 In this paper, the protoplasts, mycelium fragments and spores of Flammulina Velutipes were treated by radiation of He Ne laser respectively. It was found that the positive mutation rate and increase of yield of SOD and stability of yield of matant strains obtained from the protoplast mutagenesis were much more superior than those obtained from mycelium, fragment and spore mutagenesis. It showed that induced mutation from protoplasts by He Ne laser was an effective means of gaining excellent mutant strains of Flammulina valutipes.

【关键词】 金针菇SODHe-Ne激光诱变
【Key words】 Flammulina valutipesSODHe Ne laserinduced matation.
  • 【文献出处】 激光生物学报 ,Acta Laser Biology Sinica , 编辑部邮箱 ,2002年04期
  • 【分类号】Q947
  • 【被引频次】50
  • 【下载频次】103
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