【摘要】 目的　采用高效液相色谱法对水芹药材中绿原酸成分进行定量分析 ,建立新的质量控制方法。方法　水芹提取物甲醇溶解 ,色谱条件C18柱 ,乙腈 - 0 .4 %磷酸 (13∶87)为流动相 ,32 7nm检测。结果　水芹药材绿原酸含量为 0 .0 4 9% .结论　高效液相色谱法能精确测定水芹药材中绿原酸含量 ,可作为水芹药材质控的新方法更多还原

【Abstract】 Aim To establish a HPLC method to determing the concentration of chlorogenic acid in oenanthe javanica(OJ).Method Nova-pak HR C 18 column was used as the stationary phase,acetonitrile-0.4% phosphoric acid(13∶87) as the mobile phase,with a detection wavelength at 327nm.Results The concentration of chlorogenic acid in OJ was 0.049%.Conclusion The results showed that this method was reliable and accurate.更多还原