【摘要】 为了研究载脂蛋白E(apoE)基因调控序列 2 19(G T)多态性与人类冠心病 (CHD)及其血脂水平的关系 ,本文采用聚合酶链反应结合限制性片段长度多态性方法 ,分析了 10 8例健康人及 86例冠心病患者的 2 19(G T)基因型。PCR产物直接测序验证。我们发现冠心病组apoE 2 19(T T)基因型频率 (0 6 5 1)和T等位基因频率 (0 76 7)分别显著高于对照组 (0 4 44 ,0 6 5 3;P <0 0 5 ) ;冠心病组和对照组T T基因型者血清总胆固醇高于G G基因型者。这一结果提示apoE调控区基因多态性可能影响冠心病的发生。推测apoE 2 19(T T)基因型是冠心病的危险因子之一。更多还原

【Abstract】 A linkage between polymorphisms in apolipoprotein E promoter region and the levels of serum lipid in patients with coronary heart disease (CHD) was identified. ApoE (-219G/T) genotypes of 86 patients with CHD and 108 health subjects were examined by PCR-RFLP .The gene polymorphisms have been confirmed by sequencing. The frequency of T/T genotype was significantly higher in CHD vs control group (0.651 vs 0.444, P<0.05). The frequency of T allele was significantly higher in patients than that in control group (0.767 vs 0.653, P<0.05). The apoE promoter -219(G/T) polymorphisms influenced obviously the serum lipid levels. The concentrations of TC, and LDL-C among different genotype groups were significantly different, and the level of TC , LDL-C in T/T group was higher than that in G/G group (P<0.05). The result suggested that apoE gene promoter -219(G/T) polymorphims was closely related to serum lipid. T/T genotype of apoE promoter -219(G/T) polymorphisms may be a risk factor for coronary heart disease among Chinese.更多还原