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16SrRNA寡核苷酸探针原位杂交法在粪便微生物研究中的应用

Advances in Identification and Quantification Bacteria in Feces by in Situ Hybridization with Specific 16SrRNA-targeted Oligonucleotide Probes

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【Author】 ZHANG Mingtao1,2, GU Xianhong1, YANG lin2, LIANG HONGXING3(1.Institute of Animal Sciences, CAAS, Beijing 100094, China; 2.College of Animal Sciences and Technology, South China Agriculture University Guangzhou 510642, China; 3.Wenshi Group, Guangdong, Xinxing 527442, China)

【机构】中国农业科学院畜牧研究所；华南农业大学动物科技学院；温氏集团北京　100094；广东广州　510642；北京　100094；广东新兴527442；

【摘要】 16SrRNA寡核苷酸探针(以下简称16SrRNA探针)原位杂交法,可简单、快速和准确地对粪便微生物进行定性和定量测定,本文对该方法及其应用作一综述。更多还原

【Abstract】 The paper reviewed recent advances in molecule biological methods which were applied to identify and quantify bacteria in feces. In situ hybridization (ISH) method with specific 16SrRNAtargeted oligonucleotide probes and it operation emphasized here is a new method, which is easy, fast and accurate and must has a good application in the future.\;更多还原

【关键词】微生物；原位杂交； 16SrRNA探针；
【Key words】 bacteria； in situ hybridization； 16SrRNA-targeted oligonucleotide probes；

【基金】国家自然科学基金资助项目(30170687)

【文献出处】家畜生态 ,Ecology of Domestic Animal , 编辑部邮箱 ,2002年04期

【分类号】S852.6
【被引频次】9
【下载频次】255

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