【摘要】 目的研究丝氨酰 -天冬氨酰 -谷氨酸 (SDE)负电性三肽仿生吸附剂对低密度脂蛋白 (LDL)的吸附。方法以聚丙烯酰胺微球为载体 ,SDE三肽为配体 ,通过两种方法将配体固定到载体上制成吸附剂。1 )戊二醛法 :通过用戊二醛及氰基硼氢钠活化氨乙基化的聚丙烯酰胺微球载体进而偶联上SDE配体。2 ) 1 -乙基 - 3- ( 3-二甲基氨丙基 ) -碳二亚胺 (EDC)法 :先以EDC为偶联剂将己二胺引入聚丙烯酰胺微球载体的碱水解衍生物中 ,再用EDC将SDE配体偶联到载体上。最后用体外静态吸附法来检测吸附剂的吸附效果。结果用两种方法制备的吸附剂对LDL都有一定的亲和吸附能力 ,在吸附体系中有高浓度的Ca2 +存在时 ,效果更好 ;用戊二醛固定化配体制成的吸附剂的吸附效果较用EDC偶联配体制成的吸附剂好。结论SDE三肽对LDL有一定的亲和吸附能力 ,这为进一步研发仿生的LDL吸附剂提供实验依据更多还原

【Abstract】 Objective To study the adsorbability of tripeptide serine aspartic glutamic acid (SDE) for low density lipoprotein(LDL). Method Two methods were used to couple the ligands,SDE, to the solid supports polyacrylamide (PAM) beads, for preparing adsorbents. 1)The SDE ligands were immobilized on the solid support through activating amine groups on the aminoethyl derivatives of PAM beads with glutaraldehyde and NaCNBH 3. 2)Hexanediamine was grafted onto the carboxyl derivatives of PAM beads by 1 ethyl 3 (3 dimethylaminopropyl) carbodiimide (EDC), and then the SDE ligands were coupled to carboxyl derivatives of PAM beads graft hexanediamine by EDC. The adsorbability of adsorbents was determined in vitro. Result The adsorbents prepared by the two methods mentioned above could adsorb selectively LDL. The adsorbing efficacy was much better in the presence of Ca 2+ . The adsorbent whose ligands were immobilized on PAM beads by glutaraldehyde had better adsorbability than by EDC. Conclusion SDE can adsorb selectively LDL, which provide experimental evidence for developing advanced bionic adsorbent for LDL.更多还原