【摘要】 目的　建立一种快速简便提取嗜麦芽黄单胞菌 (X anthomonas maltophilia)基因组 DNA的方法。方法　用去污剂 SDS和 CTAB破坏细胞膜结构 ,使胞内核酸释放 ,用酚 /氯仿 /异戊醇去除蛋白质 ,异丙醇沉淀DNA,TE溶解 DNA。结果　提取的 DNA琼脂糖电泳图谱与 Hauben及 Dufresne方法提取的 DNA图谱相同 ,DNA含量为 34 2～ 388μg/ m l,OD2 60 / OD2 80 为 1.88～ 1.90。 DNA能被 Eco R 酶切消化。结论　本方法能快速简便提取 X.maltophilia DNA,提取的 DNA含量较高、纯度较好 ,可用于酶切分析、构建文库及 PCR操作。更多还原

【Abstract】 Objective To develop a rapid approach in extracting the genomic DNA from the X maltophilia. Methods We used detergent SDS and CTAB to destroy the structure of cell membrane and liberate the cellular nucleic acids, then removed the proteins using phenol/chloroform/isoamyl alcohol, precipitated the DNA using isopropanol, and finally dissolved the DNA using TE buffer. Results The DNA map of agarose electrophoresis was the same as those generated from the Hauben’s and Dufresne’s approaches. The DNA concentration was 342-388 μg/ml. The OD 260/OD 280 was 1.86-1.90. The DNA was able to be digested by the EcoRⅠ.Conclusion This approach can extract the DNA from X maltophilia rapidly. The concentration and purity of the DNA is good enough for the digest by restriction endonucleases, the establishment of genomic library, and the PCR analysis.更多还原