【摘要】 目的 :探讨牙源性角化囊肿的组织由来。方法 :将小鼠胎仔的牙板与牙乳头、口腔粘膜上皮与牙乳头 ,以及牙胚制成移植片 ,分别移植到同系成鼠的肾被膜下 ,定期回收组织片 ,进行组织病理学观察。结果 :发现 5 8例移植片中 ,4 1例形成角化囊肿 ,4 5例伴有牙体组织形成。结论 :实验产生的角化囊肿在组织学上与人的牙源性角化囊肿十分相似 ,可以为进一步研究该囊肿提供一个较理想的动物模型 ;证实了牙源性上皮可以发生角化 ;提示了牙板、口腔粘膜上皮和外釉上皮可能是该囊肿的组织由来之一。更多还原

【Abstract】 Objective:The purposes of the present animal experiment were to investigate the histogenesis of odontogenic keratocyst.Methods:A total of 17.5_day_old C\-3H mouse embryos were removed from pregnant females and placed in 2% bovine serum in Hanks solution. Under a dissecting microscope, the mandibular first molar tooth germs with the dental lamina and oral epithelia were cut from these embryos. The following 3 kinds of grafts were prepared, including recombinant of the dental lamina and dental papilla, recombinant of the oral epithelium and dental papilla, and tooth germ. Three_month_old syngenic male mice were anaesthetized by intraperitoneal injection of pentobarbital sodium and these grafts were transplanted under the left renal subcapsular spaces of the mice at the rate of one graft per capita. The grafts were harvested at times ranging from 1 to 24 weeks and were fixed in 10% neutral buffer formalin and demineralized in 5% formic acid at 4 ℃ for 12 h. The specimens were processed for histological examination. Serial sections were made, stained with hematoxylin and eosin, examined using a light microscope.Results: Three kinds of grafts grew into cysts lined by parakeralinized or orthokeratinized stratified squamous epithelia. The histopathological features of these cysts were the similar to those seen in the odontogenic keratocyst of human. Of these 58 cases of grafts, keratocyst_like lesions were formed in 41 cases and, dental tissue formation were found in 45 cases.Conclusion: These results suggest that the dental lamina, the oral epithelium and external enamel epithelium of enamel organ are the sources of the odontogenic keratocyst. The dental lamina and the oral epithelium, when recombinant with the dental papilla, have compensatory potential of dental tissue formation.\;更多还原