【摘要】 作为先进的电生理技术 ,膜片钳在生命科学研究中已成为一种愈来愈重要的技术。特别是膜片钳与其它生化技术例如 Fura- 2显微荧光测钙技术、单细胞逆转录多聚酶链式反应技术等的有机结合 ,使其应用更加广泛。本文简要叙述了膜片钳的基本原理、常用技术及其在生命化学中的应用更多还原

【Abstract】 As an advanced electrophysiological technique, the patch clamp technique has become one of the most important methods in the research field of life sciences. It is more powerful when combining with other techniques, such as the fura 2 microfluorometry (for measuring the concentration of Ca 2+ ) and single cell reverse transcription of RNA followed by the polymerase chain reaction (RT PCR). In this article, the general mechanism and the models of the patch clamp technique and its applications to life chemistry are summarized.更多还原