【摘要】 目的以斑点杂交法及PCR定量检测法评价干扰素抗HBV的疗效。方法90例慢性乙肝患者干扰素治疗前后均同时用两种方法检测血清HBVDNA。结果HBVDNA定量在总体上呈下降趋势。治疗后 2个月末与 4个月末其病毒拷贝数分别为 5 .83± 1.0 5 ,5 .75± 1.2 1,与治疗前 6 .74± 1.6 8相比差异均有显著性 (P <0 .0 5 )。HBVDNA定量PCR较斑点杂交更能反映干扰素治疗过程中患者外围血内病毒拷贝数的增长。结论两种方法均能灵敏地反映干扰素抗HBV的疗效 ,但在实际中定量PCR更灵敏。更多还原

【Abstract】 ObjectivesTo detect HBV-DNA by dot hybridization and quantitative PCR and appraise the therapeutic efficacy of interferon treatment.MethodsSerum samples from ninety patients with chronic HBV hepatitis were examined by two methods of HBV-DNA detection before and after interferon treatment. ResultsQuantitative analysis of HBV-DNA revealed a decreasing tendency of viral copies as a whole. Quantitative PCR of HBV-DNA reflected the increase of viral copies in patient’s peripheral blood more accurately than dot hybridization during the course of interferon therapy.ConclusionTwo methods of HBV-DNA detection can depict distinctly the therapeutic efficacy of interferon to antagonize HBV, but quantitative PCR of HBV-DNA is more sensitive in practical application.更多还原