【摘要】 目的:观察甲胎蛋白对细胞增殖的影响。方法:用从人脐带血清中提取的甲胎蛋白,作用于体外培养的NIH3T3细胞和人肝癌Bel7402细胞,运用MTT染色计数法,细胞周期时相测定和3H脱氧胸腺嘧啶核苷(3H-TdR)参入法等指标检测细胞增殖状况。结果:甲胎蛋白(10～80mg·L-1)作用24h后,对NIH3T3细胞、人肝癌Bel7402细胞均有不同程度的促增殖作用,甲胎蛋白的单克隆抗体能阻断这种作用。结论:甲胎蛋白是促进某些新生细胞或肿瘤细胞增殖的内源性物质。更多还原

【Abstract】 Objective:To observe the effects of alpha-fetoprotein(AFP)on the cell prolifer-ation.Method:AFP was collected and purified from human cord serum and incubated with NIH3T3cells and human hepatoma Bel7402cell which cultured in vitro.The methods of MTT count,the anal-ysis of cell cycle phase and 3 H-thymidine deoxyribonucleotide( 3 H-TdR)incorporation were used for check the conditions of cell proliferation.Results:AFP(10～80mg ·L -1 )could enhance the proliferations of both NIH3T3cells and Bel7402cell24hours after incubation.The monoclone antibody of AFP could block these effects.Conclusion:AFP could be an endogenous substance which can enhance the proliferation of some neoplastic or tumor cells.更多还原