【摘要】 目的 :测定河南食管癌高发区居民正常食管粘膜上皮和各级癌前病变组织细胞周期蛋白cyclinE和p2 7的表达。方法 :食管癌高发区 14 2例食管纤维内镜活检组织 ,采用免疫组化卵白素 生物素 过氧化物酶复合物(ABC)法和组织病理学方法 ,分析 14 2例食管纤维内镜活检组织cyclinE和p2 7的表达。 结果 :14 2例食管纤维内镜活检组织 ,共发现 33例正常食管上皮 (NOR) ,4 6例基底细胞过度增生 (BCH) ,2 6例间变 (DYS)和 4 0例原位癌组织(CIS)。正常上皮和各级癌前病变组织均出现不同程度的cyclinE和 p2 7的表达。随着病变发展 ,从NOR→BCH→DYS→CIS ,cyclinE的表达逐渐增强 (P <0 .0 1) ,免疫阳性率分别为 2 3% (6 /2 6 ) ,2 5 % (11/4 0 ) ,4 6 % (6 /13) ,5 5 %(2 4 /4 4 )。而 p2 7的表达逐渐降低 (P <0 .0 1) ,免疫阳性率分别为 5 3% (18/33) ,35 % (16 /4 6 ) ,2 7% (7/2 6 ) ,33%(13/4 0 )。结论 :cyclinE和p2 7表达异常是食管癌变早期重要的分子改变 ,cyclinE的高表达和 p2 7的低表达可能是导致病变持续向癌的方向发生和发展的机制之一更多还原

【Abstract】 Aim: To characterize the changes of cyclinE and p27 expression in esophageal normal epithelial tissue and different precancerous lesions and to correlate the changes with lesion progression from the subjects at high incidence area for esophageal cancer in Henan. Methods: Immunohistochemical and histopathological(ABC)method were used to determine the expression of cyclinE and p27 on 142 esophageal biopsies. Results: Histopathological examination showed that, of the 142 biopsies, there were 33 as normal esophageal epithelia (NOR), 46 as basal cell hyperplasia (BCH), 26 as dysplasia (DYS) and 40 as carcinoma in situ (CIS). Positive immunoreactivity with different degree for cyclinE and p27 was observed in the normal tissues and the tissues with different precancerous lesions. With the lesions progressed from NOR to BCH to DYS to CIS, the positive immunostaining rates for cyclinE increased apparently ( P <0.01), and were 23%(6/26), 25%(11/40), 46%(6/13) and 55%(24/44), respectively; in contrast, the positive immunostaining rates for p27 decreased with the lesions progressed( P <0.05), and were 53%(18/33), 35%(16/46), 27%(7/26) and 33%(13/40), respectively. Conclusion: Abnormal expression of cyclinE and p27 may be one of the important molecular changes in the early stage of esophageal carcinogenesis, and the high expression of cyclinE and low expression of p27 may be one of the mechanisms to drive the mild lesion towards carcinogenesis.更多还原