【摘要】 目的 :探讨PTEN蛋白在食管鳞癌和贲门腺癌组织中表达变化及其与细胞分化程度及淋巴结转移的关系。方法 :采用免疫组化卵白素 生物素 过氧化物酶复合物 (ABC)法和组织病理学方法 ,分析河南食管癌高发区林州市 14 1例食管鳞癌和贲门腺癌患者PTEN的表达状况。结果 :食管鳞癌和贲门腺癌PTEN阳性率分别为 4 7%(34/73)和 4 3% (2 9/6 8)。随着肿瘤分化程度的升高 ,PTEN蛋白的表达有增高的趋势 ,淋巴结转移组PTEN免疫阳性率较无淋巴结转移组高 ,但统计分析未发现PTEN免疫阳性反应率与分化程度和淋巴结转移间存在相关性(P均大于 0 .0 5 )。结论 :河南食管癌高发区食管鳞癌和贲门腺癌存在一致的PTEN蛋白变化 ,提示 2者可能具有相似的发病因素和分子机制更多还原

【Abstract】 Aim: To characterize the changes of PTEN expression in esophageal squamous cell carcinomas (SCC) and gastric cardia adenocarcinoma (GCA) and to correlate the changes with differentiation and lymph node metastasis.Method: PTEN expression was determined with immunohistochemical method (ABC) on 141 patients with SCC and GCA from Henan, the high incidence area for SCC.Results: The positive immunostaining rate for PTEN in SCC and GCA was 47%(34/73) and 43%(29/68), respectively. The increasing tendency for PTEN immunostaining was observed with differentiation, and PTEN immunostaining rate was higher in the patients with lymph node metastasis than those without. However, the difference was not significant( P >0.05).Conclusion: The high coincidence of PTEN positive immunostaining in both SCC and GCA indicates that there might be similar risk factors and molecular basis for SCC and GCA in the high risk area.更多还原