【摘要】 目的 :制备高表达GM CSF的淋巴瘤细胞系 ,观察淋巴瘤细胞转染GM CSF基因后生物学特性的改变。方法 :将小鼠GM CSF真核表达质粒用电穿孔法导入小鼠淋巴瘤细胞系RMA ,有限稀释法制备单个细胞克隆 ,经RT PCR、骨髓祖细胞增殖实验和集落形成实验筛选相对高表达GM CSF的RMA克隆 ,并观察其生物学特性的改变。结果 :获得了高表达GM CSF的RMA细胞系 ,其同基因动物体内致瘤性降低。结论 :淋巴瘤细胞系RMA转染GM CSF基因后致瘤性降低。更多还原

【Abstract】 Aim: To select high GM-CSF expressing mouse leukemia cell lineage RMA after transfected with GM-CSF gene. Methods: Eukaryotic GM-CSF expressing plasmid was transfected into RMA cells by electroporation. After screening by G418 and cloning by limited dilution, a relative GM-CSF high expressing cell clone was selected by RT-PCR, hematopoietic progenitor cell proliferative assay and hematopoietic progenitor cell colony formation assay. Results: A GM-CSF high expressing cell lineage RMA-GM was selected whose ability of tumor formation was lower than RMA. Conclusion: The in vivo growth ability of lymphoma cell line RMA is suppressed after transfected with GM-CSF gene.更多还原