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8-Br-cAMP对人肺腺癌细胞P53蛋白表达和端粒酶活性的影响

Influences of 8-Br-cAMP on the telomerase activity and P53 expression in the human lung adenocarcinoma cell A549 line

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【Author】 WANG Aiping 1), ZHANG Ting 2), ZHU Linlin 3) 1)Department of Clinical Laboratory, the Affiliated Anzhen Hospital,Capital Medical University, Beijing 100029 2)Department of Biochemistry,Henan Traditional Chinese Medical College, Zhengzhou 4500

【机构】首都医科大学附属北京安贞医院检验科；河南中医学院生物化学教研室；濮阳县人民医院检验科北京100029；郑州450003；濮阳457002；

【摘要】目的 :探讨 8 Br cAMP对人肺腺癌细胞株A5 49细胞端粒酶活性和P5 3蛋白表达的影响。方法 :用终浓度分别为 2 0 μmol/L及 6 0 μmol/L的 8 Br cAMP处理人肺腺癌细胞株A5 49细胞 ,以未处理者为空白对照。采用PCR ELISA法检测端粒酶活性 ,用免疫荧光标记法测定P5 3蛋白的表达。结果 :8 Br cAMP作用后的A5 49细胞 ,端粒酶活性明显降低 ,P5 3蛋白表达明显减少。结论 :8 Br cAMP能抑制肺腺癌细胞的端粒酶活性和P5 3蛋白的表达。更多还原

【Abstract】 Aim:To investigate the influence of 8-Br-cAMP on the telomerase activity and P53 expression in the human lung adenocarcinoma cell line(A549).Methods:The human 1ung adenocarcinoma cell line(A549)was treated with 8-Br-cAMP (20 μmol/L,60 μmol/L),and the human lung adenocarcinoma cell untreated was taken as sham control. P53 expression and the telomerase activity were detected by immunohistochemisty and PCR-ELISA method in the course of differentiation of A549 cells.Results:Both P53 expression and the telomerase activity were signficantly decreased in the A549 cell line treated with 8-Br-cAMP.Conclusion:8-Br-cAMP could inhibit the telomerase activity and the P53 expression of A549 cells.更多还原

【关键词】肺肿瘤；腺癌； 8-Br-cAMP；端粒酶； P53蛋白；
【Key words】 lung neoplasm； adenocarcinoma； 8-Br-cAMP； telomerase； protein P53；

【文献出处】郑州大学学报(医学版) ,Journa of Henan Medical University , 编辑部邮箱 ,2002年04期

【分类号】R734.2
【被引频次】2
【下载频次】29

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