【摘要】 目的 :探讨经肝动脉移植微囊化新生猪胰岛细胞后 ,移植物生理功能以及副反应的特点。方法 :化学药物诱导制作犬的糖尿病模型后 ,将分离、纯化及微囊化的新生猪胰岛细胞经肝动脉移植入糖尿病犬的肝内 ,测定移植前后糖尿病犬的胰岛素用量、血糖水平以及葡萄糖刺激血清C肽、肝功能及CD4 /CD8的变化 ,并于移植 6月后对移植受体肝、胰进行病理学检查。结果 :移植后模型犬血糖逐步下降至正常水平 ,胰岛素用量减少 ,移植 6月后 2犬停用胰岛素 ,并维持血糖正常达 180d之久 ;移植后肝检查无明显变化 ,胰腺检查见凋亡胰岛无再生现象。结论 :微囊化的新生猪胰岛细胞经肝动脉移植入糖尿病犬的肝内 ,能纠正异种动物犬糖尿病模型的高血糖状态 ,不发生明显副作用更多还原

【Abstract】 Objective To evaluate the physiologic features and probable side effects of transarterial intrahepatic xenotransplantation of microencapsulated newborn porcine islets. Methods Six artificial diabetic canines were transplanted intrahepatically with 6～9×10 5 encapsulated newborn porcine islets without immunosuppression. Before and after the transplantation, the serum C peptide, glucose tolerance test, and dosage of insulin were measured. Samples of the livers and pancreases were examined pathologically 6 months after the operation. Results After the transplantation of microencapsulated porcine islets, the serum C peptides were elevated significantly. The plasma glucose returned to normal gradually. The dosage of insulin decreased. Six months after the operation, the usage of insulin in 2 dogs was stopped and the normal plasma glucose was kept for 180 days. The pathologic examination showed the xenografts located in the interlobular artery of the livers and the livers of canines were not damaged. Conclusion The transplantation may correct the high plasma glucose level of diabetic canines without immunosuppression and has no side reactions.更多还原