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受辐照窄颖赖草花粉DNA进入小麦胚囊的电镜自显影证据及杂种原位杂交鉴定
EM AUTORADIOGRAPHY EVIDENCE FOR IRRADIATED POLLEN DNA OF Leymus angustus ENTERING WHEAT OVARY AND GISH OF ITS HYBRIDS
【摘要】 采用3H 胸腺嘧啶标记窄颖赖草 (Leymusangustus)花粉结合电镜自显影的方法证明受辐照窄颖赖草花粉DNA进入了小麦胚囊 ,运用基因组原位杂交技术证明所得杂种为真杂种 ,经辐照花粉获得的普通小麦J 1 1与窄颖赖草杂种中窄颖赖草染色体发生了数目和结构变异
【Abstract】 It was proved that DNA of irradiated pollen of Leymus angustus entered wheat ovary by means of labeling pollens of L.angustus with 3H\|TdR and electronic microscopic autoradiography.Genomic in situ hybridization analysis on wheat× L.angustus hybrids showed that chromosome number variation and structural aberration of L.angustus took place in the hybrids recovered after pollinating with irradiated pollens of L.angustus.
【关键词】 普通小麦;
窄颖赖草;
辐照花粉;
电镜自显影;
基因组原位杂交;
【Key words】 Triticum aestivum L.; Leymus angustus; EM autoradiography; GISH;
【Key words】 Triticum aestivum L.; Leymus angustus; EM autoradiography; GISH;
【基金】 国家自然科学基金资助项目 (39770 4 97)
- 【文献出处】 核农学报 ,(牠丁文)acta Agriculturae Nucleatae Sinica , 编辑部邮箱 ,2002年03期
- 【分类号】Q945
- 【被引频次】6
- 【下载频次】68