【摘要】 目的　探讨抗转化生长因子β1(anti-TGF - β1)对瘢痕成纤维细胞增殖及胶原合成的影响及意义。方法　用电镜观察anti-TGF - β1作用后瘢痕成纤维细胞的形态学变化 ,以及用MTT法和3 H -脯氨酸掺入等方法检测anti-TGF - β1对体外培养的瘢痕成纤维细胞的增殖及胶原蛋白合成的影响。结果　anti -TGF - β1能明显影响成纤维细胞的超微结构 (细胞核、线粒体、粗面内质网等 )。anti -TGF - β1能抑制成纤维细胞的增殖和胶原蛋白的合成 (P <0 .0 1) ,在一定范围内 (10 μg/ml)呈剂量 -效应关系。抑制作用在 10 μg/ml时最大 ,抑制率达 72 %。结论　anti-TGF - β1能中和TGF - β1,促进成纤维细胞增殖和胶原合成的作用 ;提示anti-TGF - β1的应用可能为临床瘢痕的治疗提供新的途径。更多还原

【Abstract】 Objective To investigate the significance and the influence of anti-transforming growth factor-β 1 on fibroblast proliferation and collagen synthesis. Methods Electron microscope (EM) was employed to study the morphological changes in fibroblast after the application of anti -TGF-β 1.The effects of anti -TGF-β 1 on the proliferation and collagen synthesis of the fibroblasts were investigated by means of 3H- proline incorporation and MTT reaction .Results It was found that anti-TGF-could significantly affect the ultrastructure of fibroblast and inhibit its proliferation and its collagen synthesis in vitro (P<0.01) in a manner of dose-effect relationship. The rate of inhibition (72%) is the highest in dose of 10μg/ml.Conclusion Fibroblast proliferation and collagen synthesis could be depressed by anti -TGF-β 1 in vitro by means of neutralization. It seems possible to provide a new way for the treatment of scar.更多还原