【摘要】 菌种的质量及其稳定性在生物工程产品发酵中起着至关重要的作用。本文重点考察了重组人粒细胞集落刺激因子(G—CSF)菌种在 LB 固体培养基上(4℃条件下)的稳定性。试验表明该菌种可在 LB 固体培养基上稳定传代三代(60天),从而大大简化了重组人粒细胞集落刺激因子(G—CSF)生产用菌种的制备过程。更多还原

【Abstract】 The quality and stability of E.coli producing Recombinant Human Granulocyte Colony Stimulating Factor are very important in the bioproducts fermentation.This essay mainly studies the stability of the E.coli on solid LB medium under 4℃.We conclude that the E.coli can be stable for 3 generations(60days).This result greatly simplifies the preparation of the produc- tion E.coli and has great meaning in production.更多还原