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塔玛亚历山大藻对栉孔扇贝胚胎和早期幼虫的影响

The effects of the dinoflagellate of Alexandrium tamarense on early development of Chlamys farreri

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【作者】 颜天傅萌王云峰于仁诚李钧周名江

【Author】 YAN Tian, FU Meng, WANG Yunfeng, YU Rencheng, LI Jun, ZHOU Mingjiang\ (Institute of Oceanology, The Chinese Academy of Sciences, Qingdao\ 266071)

【机构】 中国科学院海洋研究所中国科学院海洋研究所 青岛266071青岛266071青岛266071

【摘要】 塔玛亚历山大藻Alexandriumtamarense(藻株ATHK)能抑制栉孔扇贝Chlamysfarreri受精卵的孵化和早期D型幼虫的存活 .实验发现 ,暴露于密度为 10 0和 5 0 0cells mL的塔玛亚历山大藻中 ,36h后 ,扇贝受精卵的孵化率分别为对照组的 30 %和5 % .在实验范围内 ,三角褐指藻和赤潮异弯藻对受精卵孵化无影响 ,而另一塔玛亚历山大藻藻株ATCI0 2对受精卵孵化有显著影响 .指数生长前期时的藻对受精卵的毒性最大 .原肠期之前各阶段的胚胎对塔玛亚历山大藻毒性最敏感 .在密度为 30 0 0cells mL以上的塔玛亚历山大藻中 ,早期D型幼虫存活和活力开始显著下降 ,在密度为 5 0 0 0cells mL藻中暴露 2 0d或 10 0 0 0cells mL藻中暴露 14d后 ,D型幼虫的存活率为 0 .塔玛亚历山大藻产生的毒性物质为非PSP毒素的一类未知毒素

【Abstract】 The inhibition of the dinoflagellate Alexandrium tamarense on the fereilized egg hatching success and larval survival of the scallop(\%Chlamys ferreri\%) was studied. The hatching rate of \%C. farreri\% was only 30% when the fertilized eggs were exposed for 36 hours to \%A. tamarense\% cells at a concentration of 100 cells/mL, and further dropped to 5% with the concentration of 500?cells/mL. There was no such effects when the fertilized scallop eggs exposed to other two algal species, the diatom \%Phaeodactylum tricornutum\% and raphidophyte \%Heterosigma carterae\%. The alga at early exponential stage had the strongest effect on egg hatching and \%algal\% toxin(s) influenced the developmental stage before blastula most sensitively. The alga also deliriously influenced the larvae at early D shape stage of the scallop. The survival rate began to decrease significantly after exposed for 6 days at concentration of 3?000?cells/mL and above; no larvae could survive after 14 day exposure to A. tamarense at 10?000?cells/mL or 20 day at 5?000?cells/mL. As PSP is an internal toxin of algal cells and algal cells are un able to be ingested at these stages of the scallop,thus the experimental results indicated that there may be produced novel substances other than PSP from \%A. tamarense\%.

【基金】 国家自然科学基金 (3995 0 0 0 1;495 76 30 1;2 0 1770 2 3;4990 6 0 0 7) ;山东省科技攻关专项 ;中国科学院知识创新工程 (KZCX2 2 0 6 );院长基金 ;国家重点基础研究项目 2 0 0 1CB 40 970 9
  • 【文献出处】 环境科学学报 ,Acta Scientiae Circumstantiae , 编辑部邮箱 ,2002年02期
  • 【分类号】X171.5
  • 【被引频次】17
  • 【下载频次】130
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