【摘要】 通过控制培养基中Mn2 +的浓度获得只含有木质素过氧化物酶 (LiP)或锰过氧化物酶 (MnP)的单一酶培养物 ,用于比较LiP和MnP对染料降解脱色的条件和能力的差异。结果表明 ,LiP对橙Ⅰ降解脱色的最佳条件为H2 O2 0 15mmol L ,pH 3 0 ,温度 4 0℃ ;MnP为H2 O2 0 15mmol L ,pH 3 5,温度 4 0℃。最佳条件下 ,考察LiP和MnP作用下染料浓度和染料类型对脱色的影响 ,发现LiP作用下脱色率明显高于MnP作用下的脱色率 ,表明LiP可作用的染料浓度比MnP高 ,可作用的染料范围比MnP大。由此认为LiP具有比MnP更强的脱色能力更多还原

【Abstract】 The cultures only containing lignin peroxidase (LiP) or manganese peroxidase (MnP) were obtained by controlling the concentration of Mn\+\{2+\} in medium.The single enzyme culture was used to investigate the decoloration conditions and ability of LiP and MnP on dyes.The optimal decoloration condition of Orange Ⅰ by LiP was H\-2O\-2 0 15 mmol/L,pH 3\^0 and temperature 40 ℃,while it was H\-2O\-2 0\^15 mmol/L,pH 3 5 and temperature 40 ℃ by MnP.When the effect of concentration and type of dyes were studied under optimal conditions,higher decoloration was obtained when LiP was used.It was concluded that LiP could be applied in higher concentration and wider range of dyes than MnP could,which showed that the decoloration ability of LiP was much higher than that of MnP.更多还原