【摘要】 目的探讨胶质母细胞瘤中p16基因、视网膜母细胞瘤基因 (retinoblastomagene,RB)、细胞周期蛋白D1(cyclinD1)基因、细胞周期蛋白依赖性激酶 4 (cyclindependentkinase 4 ,CDK4 )基因表达的异常及其与细胞增殖活性之间的关系。方法应用流式细胞仪检测 32例胶质母细胞瘤标本中p16、RB、cyclinD1、CDK4基因蛋白表达的相对含量。结果胶质母细胞瘤中 p16、RB基因蛋白表达量较正常脑组织降低 ,cyclinD1、CDK4基因蛋白表达量较正常脑组织升高 ,二者的异常可使细胞发生过度增殖。结论 p16、RB基因蛋白表达量降低和cyclinD1、CDK4基因蛋白表达量升高是胶质母细胞瘤发生发展的重要因素之一更多还原

【Abstract】 ObjectiveTo explore the abnormalities of expression of p16,retinoblastoma(RB),cyclinD1,cyclin dependent kinase 4(CDK4)genes and the relationship between these abnormalities and the cell proliferation in glioblastomas(GBMs). MethodsThe expression of p16 gene and cyclinD1 gene was measured by flow cytometry in 32 cases of GBMs. ResultsThe amounts of the expression of p16 gene in GBMs are lower than those in normal brains,and the amounts of the expression of cyclinD1 gene are higher. The abnormalities of the two proteins may cause the over proliferation of GBMs cells. ConclusionThe abnormalities of the expression of p16 gene, cyclinD1 gene are the important factors for the development of GBMs.更多还原