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RAPD分子标记技术应用于家蚕品种纯度检测的研究
Study on the purity detection of mulberry silkworm ( Bombyx mori ) varieties by applying the RAPD molecular marking technique
【摘要】 对家蚕品种“932”、“75 32”、芙蓉、湘晖等的原种 ,“932”×芙蓉、“75 32”×湘晖的杂交原种 ,(“932”×芙蓉 )× (“75 32”×湘晖 )F1杂交种进行RAPD分子标记纯度检测筛选 ,经过 180个随机引物的筛选 ,获得可用于判别“932”×芙蓉、“75 32”×湘晖杂交原种、(“932”×芙蓉 )× (“75 32”×湘晖 )四元杂交种纯度的分子标记 ,初步建立了从DNA提取到纯度判别的技术方法。
【Abstract】 By applying RAPD molecular marking technique and through the selection of 180 random primers,a technical method was preliminary established to extract the DNA and to detect the purity of mulberry silkworm ( Bombyx mori ) eggs of parental varieties named 932,7532,Furong and Xianghui,parental hybrids named 932×Furong and 7532×Xianghui,and the quaternary hybrid named (932×Furong)×(7532×Xianghui).
【关键词】 家蚕蚕种;
杂种纯度;
RAPD技术;
检测;
【Key words】 mulberry silkworm egg; hybrid purity; RAPD technique; detection;
【Key words】 mulberry silkworm egg; hybrid purity; RAPD technique; detection;
【基金】 广东省自然科学基金 ( 0 0 0 5 98)资助
- 【文献出处】 湖北农业科学 ,Hubei Agricultural Sciences , 编辑部邮箱 ,2002年03期
- 【分类号】S882
- 【被引频次】14
- 【下载频次】52