【摘要】 簇毛麦和中间偃麦草是小麦改良的重要抗源 ,为了对导入的外源染色体及片段进行有效鉴定 ,应用分带和双色荧光原位杂交 ,将 2 5S 5 8S 18SrRNA、 5SrRNA基因分别定于簇毛麦染色体 1V短臂和 5V短臂上。分别在中间偃麦草的 3对、 4对染色体上观察到 2 5S 5 8S 18SrRNA和 5SrRNA基因 ,其中有 2对染色体在其短臂上有两种核糖体RNA基因更多还原

【Abstract】 Haynaldia villosa(2n=14,VV) and Thinopyrum intermedium (2n=42,E 1E 1E 2E 2 StSt) are important wheat relatives providing useful resistant genes for wheat improvement.In this paper,rRNA genes coding 25S 5 8S 18S rRNA and 5S rRNA are visualized on H.villosa and Th.intermedium chromosomes by double color fluorescence in situ hybridization.The 25S 5 8S 18S rRNA genes are localized on the short arm of 1V chromosome,and 5S rRNA genes are on the short arm of 5V chromosome of H.villosa. On the other hand,three pairs of the 25S 5 8S 18S rRNA genes and four pairs of the 5S rRNA genes are observed on the Th.intermedium chromosomes,in which 2 pairs of chromosomes have both rRNA genes on their short arms.更多还原