【摘要】 从采集的家庭污水样品中 ,分离筛选出一株暂定名为假丝酵母 (Candidasp .Jw4 )的菌株 .该菌株在含油的筛选琼脂平皿上生长良好 ,具有丰满的假菌丝 .最佳产酶条件是 :发酵培养基 (g/L)为菜籽油 30 ,玉米浆 30 ,酵母粉 2 ,K2 HPO4 5 ,MgSO4 ·7H2 O 1和CaCl2 0 .2 ,pH 6 .5 ,2 8℃ .Jw4脂肪酶活力最适温度为4 2℃、最适 pH 8.2 .Jw4既可利用植物油也可降解动物油 .在菜籽油 (皂化值 174 .74 )为 2 5 0 g/L或猪油 (皂化值 185 .10 )为 2 5 0 g/L的反应液中 ,加入粗酶液 ,分别使反应液酶浓度为 10u/mL和 2 0u/mL ,pH 8.0～8.2 ,4 0℃通气振荡 12 0h ,菜籽油和猪油分别脱脂达 80 %和 73% .更多还原

【Abstract】 A strain isolated from domestic sewage was tentatively identified with Candida sp.Jw4. The optimum conditions for enzyme production were as follows:30 g/L rape oil,30 g/L Corn steep liquor,2 g/L yeast powder, 5 g/L K 2HPO 4,1 g/L MgSO 4·7H 2O and 0.2 g/L CaCl 2 with pH 6.5,at 28 ℃.Crude enzyme produced by Candida sp.Jw4 has an optimum temperature of 42 ℃,the enzyme has the highest activity at pH 8.2.The 250 g/L vegatable seed oil or 250 g/L pig oil were digested up to 80% or 73% respectively, when reaction system containing enzyme 10 or 20 u/mL,at pH 8.0～8.2,40 ℃,shaking for 120 h.更多还原