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HPLC法测定丙酮酸钙的含量
Determination of Calcium Pyruvate by HPLC
【摘要】 建立一种用高效液相色谱法(HPLC法)测定丙酮酸钙含量的方法。色谱条件为C18柱,乙腈—0.1%二环已胺和0.1%甲酸的水溶液(5:95)为流动相, 检测波长为230nm。线性范围0.08~0.8mg/mL(r = 0.9999)。
【Abstract】 A HPLC method for the determination of calcium pyruvate was developed. The chromatographic system consisted of C18 column and mobile phase of acetonitrile-aqueous solution (add 1mL dicyclohexylamine and 1mL formic acid anhydrous to 1000mL H2O)(5:95). The detection wavelength was 230nm. The calibration curve was linear from 0.08 to 0.8mg/mL with a regression coefficient of 0.9999.
- 【文献出处】 广州化学 ,Guangzhou Chemistry , 编辑部邮箱 ,2002年01期
- 【分类号】O657.72
- 【被引频次】18
- 【下载频次】337