【摘要】 目的 探讨骨形态发生蛋白、自体红骨髓、羟基磷灰石及纤维蛋白复合物的制备方法及其诱导成骨活性。方法 在大白鼠股后肌群分别植入(1)红骨髓(RBM)、(2)纤维蛋白-红骨髓复合物(FS-RBM)、(3)纤维蛋白-羟基磷灰石-红骨髓复合物(FS-HA-RBM)、(4)纤维蛋白-羟基磷灰石复合物(FS-HA)、(5)纤维蛋白-羟基磷灰石-骨形态发生蛋白复合物(FS-HA-BMP)、(6)纤维蛋白-羟基磷灰石-骨形态发生蛋白-红骨髓复合物(FS-HA-BMP-RBM),按1,2,4周三个时间点进行大体标本、组织学观察及ALP检测,比较各组间诱导成骨活性。结果 FS-HA-BMP-RBM组复合物有较多的新生软骨和骨形成。各组ALP活性比较显示,HA-FS-BMP-RBM组最强,且于第2周最高。结论 FS-HA-BMP-RBM复合物具有良好的诱导成骨能力。更多还原

【Abstract】 Objective To study method of preparation of th compounds BMP, HA, RBM and FS, and to determine their osteoinductivity.Methods Six kinds of implants were obtained i.e. A:RBM;B:FS- RBN;C:FS- HA- RBM;D:FS- HA;E: BMP - HA - RBM - FS; and F: FS - HA - BMP; and then they were implanted into the muscle pounches in the posterior - thigh of rats. The bone formation was evaluated by macroscopic and histological observations and determination of alkaline phosphatase activity after 1,2 and 4 weeks of operation. Results There were much more new cartilage and bone formation in the group implanted with BMP - HA - RBM - FS(E)than in any other groups. ALP activity was the highest in group E among all groups. Conclusion The compound of BMP - HA - RBM - FS posses much higher osteoinductive potential.更多还原